Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Hidehito Saito; Kumiko Iwabuchi; Noemi Fusaki; Fumito Ito

doi:10.3791/54375

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

从人黑色素瘤肿瘤浸润淋巴细胞诱导多能干细胞的产生

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10:03 min

November 11, 2016

DOI: 10.3791/54375-v

Hidehito Saito^1,2, Kumiko Iwabuchi³, Noemi Fusaki^4,5, Fumito Ito^3,6

¹Department of Surgery,University of Michigan, ²Department of Biochemistry II,Kanazawa Medical University, ³Center for Immunotherapy,Roswell Park Cancer Institute, ⁴DNAVEC Corporation, ⁵Department of Ophthalmology,Keio University School of Medicine, ⁶Department of Surgical Oncology,Roswell Park Cancer Institute

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该方案的目标是展示将黑色素瘤肿瘤浸润淋巴细胞重编程为诱导多能干细胞的方案。

本实验的总体目标是从黑色素瘤浸润淋巴细胞中产生诱导多能干细胞或 iPSC。这种方法可以帮助回答肿瘤免疫学领域的关键问题，尤其是癌症免疫治疗。该技术的主要优点是，从肿瘤浸润淋巴细胞产生人 iPS 细胞的能力可以为免疫治疗提供无限数量的分化程度较低的肿瘤特异性 T 细胞。

这项技术可能适用于治疗转移性黑色素瘤，因为目前临床上 T 细胞存活率低一直是适应性 T 细胞治疗的主要限制。该方法也可用于研究肿瘤微环境中的 T 细胞受体库。演示该程序的是我实验室的博士后研究员 Kumiko Iwabuchi 博士。

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发育生物学第117 诱导多能干细胞重新编程肿瘤浸润淋巴细胞 T细胞黑色素瘤仙台病毒载体人 CD3 CD28 IL-2的