Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Gatikrushna Singh; Sarah M. Fritz; Arnaz Ranji; Deepali Singh; Kathleen Boris-Lawrie

doi:10.3791/54391

Journal

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生物化学

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从细胞使用寡核苷酸定向洗脱同源RNA-蛋白质复合物的隔离

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

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生物化学

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JoVE 杂志生物化学

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

从细胞使用寡核苷酸定向洗脱同源RNA-蛋白质复合物的隔离

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9,017 Views

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10:53 min

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January 16, 2017

DOI:

10.3791/54391-v

DOI:

10.3791/54391-v

•

10:53 min

January 16, 2017

•

8996 Views

Gatikrushna Singh*¹, Sarah M. Fritz*², Arnaz Ranji², Deepali Singh³, Kathleen Boris-Lawrie^1,2

¹Department of Veterinary & Biomedical Sciences,University of Minnesota ²Department of Veterinary Biosciences,Ohio State University ³School of Biotechnology,Gautam Buddha University

Summary

This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.