14,957 Views
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10:37 min
December 09, 2016
DOI:
10.3791/54518-v
这里,我们描述协议破坏由固态研磨哺乳动物细胞在低温下,产生从所得细胞粉细胞提取物,并且在抗体偶联微米级的顺磁珠亲和捕获隔离感兴趣的蛋白质复合物。
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Cite this Article
LaCava, J., Jiang, H., Rout, M. P. Protein Complex Affinity Capture from Cryomilled Mammalian Cells. J. Vis. Exp. (118), e54518, doi:10.3791/54518 (2016).
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