JoVE Journal
Immunology and Infection
Immunology and Infection
需要订阅 JoVE 才能查看此 内容.
章节
总结概括
We describe a standard antibody staining protocol for use in microscopy to determine the membrane expression and localization of gangliosides in resting and activated human naïve CD4+ T cells. Also described are real-time PCR experiments using <40,000 cells that do not require additional low input RNA kits.