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Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
JoVE 杂志
生物学
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JoVE 杂志 生物学
Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

DOI:
10.3791/54584-v

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08:54 min

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September 17, 2016

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1Department of Biology,University of Massachusetts Boston

Chapters

  • 00:05Title
  • 00:54Preparing Cells of the Same Genotype for Sporulation
  • 02:55Preparing Cells of Different Genotypes for Sporulation
  • 04:42Sporulating Cells in a 96-multiwell Format
  • 06:29Results: Efficient Sporulation in a Multiwell Plate Format
  • 08:05Conclusion

Summary

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Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

Tags

SporulationSaccharomyces Cerevisiae96-well FormatHigh-throughput ScreeningMeiosisTiled Two Micron Plasmid LibraryHigh Copy SuppressorsRefractile SporesAerationSK1 Yeast StrainYPG PlateYPD PlateYPD CultureYPA CultureCentrifugationSporulation Media96 Multiwell Plate

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