Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Susan Wilde; Malcolm P. Logan

doi:10.3791/54618

Journal

/

发育生物学

/

在小鸡学习感应信号与候选因子浸泡珠结合防渗屏障中的应用

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

JoVE 杂志
发育生物学

This content is Free Access.

JoVE 杂志发育生物学

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

在小鸡学习感应信号与候选因子浸泡珠结合防渗屏障中的应用

Please note that all translations are automatically generated. Click here for the English version.

9,331 Views

•

08:04 min

•

November 17, 2016

DOI:

10.3791/54618-v

DOI:

10.3791/54618-v

•

08:04 min

November 17, 2016

•

12 Views

Susan Wilde¹, Malcolm P. Logan¹

¹Randall Division of Cell and Molecular Biophysics,King’s College London, Guy’s Campus

成績單

Summary

Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.