Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Volodymyr Petrenko; Camille Saini; Laurent Perrin; Charna Dibner

doi:10.3791/54673

Journal

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遗传学

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在人原代细胞培养生物钟基因表达及荷尔蒙分泌的并行测量

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

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遗传学

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JoVE 杂志遗传学

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

在人原代细胞培养生物钟基因表达及荷尔蒙分泌的并行测量

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8,249 Views

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06:53 min

•

November 11, 2016

DOI:

10.3791/54673-v

DOI:

10.3791/54673-v

•

06:53 min

November 11, 2016

•

8243 Views

Volodymyr Petrenko*¹, Camille Saini*², Laurent Perrin*¹, Charna Dibner¹

¹Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center,University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3)²Population Epidemiology Unit (UEP), Community Medicine,Geneva University Hospital

Summary

Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.