Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Nicolaas H. Fourie; Ralph M. Peace; Sarah K. Abey; LeeAnne B. Sherwin; John W. Wiley; Wendy A. Henderson

doi:10.3791/54693

Journal

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遗传学

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肠易激综合征的循环miRNA的扰动检测使用复用高通量基因表达平台

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

肠易激综合征的循环miRNA的扰动检测使用复用高通量基因表达平台

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8,213 Views

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:

10.3791/54693-v

•

10:37 min

November 30, 2016

•

9 Views

Nicolaas H. Fourie¹, Ralph M. Peace^1,2, Sarah K. Abey¹, LeeAnne B. Sherwin¹, John W. Wiley³, Wendy A. Henderson¹

¹Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, ²National Institutes of Health Research Scholar,Howard Hughes Medical Institute, ³Internal Medicine, Medical School,University of Michigan

成績單

Summary

We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.