Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

Danae Schulz; Monica R. Mugnier; Catherine E. Boothroyd; F. Nina Papavasiliou

doi:10.3791/54715

Journal

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免疫与感染

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检测布氏锥虫变体表面糖蛋白通过磁性细胞分选和流式细胞仪开关

Detection of <em>Trypanosoma brucei</em> Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

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免疫与感染

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JoVE 杂志免疫与感染

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

检测布氏锥虫变体表面糖蛋白通过磁性细胞分选和流式细胞仪开关

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8,659 Views

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09:45 min

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October 19, 2016

DOI:

10.3791/54715-v

DOI:

10.3791/54715-v

•

09:45 min

October 19, 2016

•

1 Views

Danae Schulz^1,2, Monica R. Mugnier¹, Catherine E. Boothroyd^1,3, F. Nina Papavasiliou¹

¹Laboratory of Lymphocyte Biology,Rockefeller University, ²Department of Biology,Harvey Mudd College, ³Masters School

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Summary

African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.