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Immunology and Infection

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JoVE Journal Immunology and Infection
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

Article DOI: 10.3791/54726-v • 06:45 min • November 21st, 2016
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.

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Fluorescence Assay Quantification Canine Neutrophil Extracellular Trap Release DNA Release Agonists Immunology Veterinary Medicine Neutrophil Response Agonists Of Neutrophil Extracellular Trap Induction Cell Death Technique Screening Agonists Inhibitors Neutrophil Extracellular Trap Formation 21-gauge Butterfly Needle Cephalic Vein Three-milliliter Syringe Blood Samples EDTA-containing Blood Tubes Venipuncture Atraumatic Blood Flow Poor Neutrophil Yield 50-milliliter Conical Tube Sterile Room-temperature 3%Dextran 500 Inversion Straw-colored Upper Layer Centrifuge
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