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High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Article DOI: 10.3791/54735-v • 11:30 min • October 27th, 2016
October 27th, 2016 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

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Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.

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High-fat Feeding Paradigm Larval Zebrafish Fluorescent Lipid Analog Food Intake Lipid Uptake Dynamics Quantify Dietary Lipid Intake Metabolism Gastrointestinal Biology Fluorescent Lipid Analogs Microscopy Subcellular Lipid Processing Powdered Fluorescent Lipid Analogs Chloroform Amber Glass Tubes Teflon Gasket Freezer Nitrogen Stream Ethanol Embryo Medium
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