10,873 Views
•
13:35 min
May 05, 2017
DOI:
10.3791/54874-v
此手稿描述了通过透射电子显微镜可视化用混悬剂细胞中易于使用和低成本的冷冻固定方法。
09:59
Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
Related Videos
79162 Views
07:00
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
3340 Views
09:16
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
6301 Views
11:55
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
14099 Views
10:54
Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
26606 Views
11:17
Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
33312 Views
12:52
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
16447 Views
07:33
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
10572 Views
11:45
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
9566 Views
09:03
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
5281 Views
Read Article
Cite this Article
Blancard, C., Salin, B. Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy. J. Vis. Exp. (123), e54874, doi:10.3791/54874 (2017).
Download .ris file
Copy
Share Video
.