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Immunology and Infection

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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

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This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4+ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.

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In Vitro Differentiation Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) Naive CD4+ T Cells TGF-β-containing Protocol Phenotype Analysis T Cell Immunology Molecular Level Reproducible Generation Phenotyping Modified Technique CD4 T Cell Subsets Immune Cells Visual Demonstration PBMC Isolation Density Gradient Medium Centrifugation
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