Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension

Diederik E. van der Feen; Michel Weij; Annemieke Smit-van Oosten; Lysanne M. Jorna; Quint A.J. Hagdorn; Beatrijs Bartelds; Rolf M.F. Berger

doi:10.3791/55065

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JoVE Journal Medicine

Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension

分流手术，右心导管，血管形态计量学在大鼠模型中的流量引起的肺动脉高压

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09:23 min

February 11, 2017

DOI: 10.3791/55065-v

Diederik E. van der Feen¹, Michel Weij², Annemieke Smit-van Oosten², Lysanne M. Jorna¹, Quint A.J. Hagdorn¹, Beatrijs Bartelds¹, Rolf M.F. Berger¹

¹Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital,University Medical Center Groningen, University of Groningen, ²Research and Development Facility,University Medical Center Groningen, University of Groningen

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该协议描述了一种外科手术，用于创建大鼠血流诱导的肺动脉高压（PAH）模型，以及分析该模型中主要血流动力学和组织学终点的手术。

这些程序的总体目标是创建大鼠血流诱导肺动脉高压的模型，并通过右心导管插入术和血管形态测定分析其主要血流动力学和组织学终点。我们提出了一种用于血流相关肺动脉高压的大鼠模型的创建，该模型的特征是类似于人类肺动脉高压的新型内膜型血管重塑，并且由临床公认的触发因素（即肺血流量增加）诱导。这些特征将增强该模型中的研究结果对人类疾病的转化。

我们还展示了使用定量形态测量学进行血流动力学评估和组织病理学表征的标准化方法。这些技术的影响延伸到发现和评估新的治疗策略，并允许评估对肺血流动力学和血管形态的特定影响。该实验室的显微外科医生 Annemieke Smit-Van Oosten 和 Michel Weij 将演示这些程序。

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