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Developmental Biology
多能干细胞衍生的心肌修复心肌细胞
多能干细胞衍生的心肌修复心肌细胞
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

多能干细胞衍生的心肌修复心肌细胞

Full Text
8,820 Views
06:37 min
February 3, 2017

DOI: 10.3791/55142-v

Wuqiang Zhu1, Ling Gao1, Jianyi Zhang1

1Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们提出三种新型和更有效的协议,用于人类诱导多能干细胞分化为心肌细胞,内皮细胞和平滑肌细胞,并且通过细胞注射补丁介导的细胞因子的递送组合提高移植细胞的植入的递送方法。

本视频的总体目标是介绍一种将人类多能干细胞分化为心肌细胞的技术,并展示使用这些细胞研究缺血再灌注损伤后的心肌修复。该方法可以帮助回答有关使用源自心脏细胞的人 iPS 细胞对心脏组织进行工程改造以进行心肌修复的关键问题。该程序的主要优点是它允许科学家收集纯化的心肌细胞、平滑肌细胞和内皮细胞,并用这些祖细胞生成心脏组织。

Ling Gao 是 Zhang医生在 UAB 自己的实验室的博士后,Greg Walcott 是 UAB 医院的心脏病专家。Trina 是 UAB 医院的 MRI 专家。为了将多能干细胞诱导到心肌细胞中,首先,在冷却至 4 摄氏度的生长因子还原凝胶蛋白混合物包被六孔板的孔中。

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