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线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
JoVE 杂志
生物学
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JoVE 杂志 生物学
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

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08:43 min

January 24, 2017

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08:43 min
January 24, 2017

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Summary

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线粒体可以利用在其内的膜(Δ 膜电势 )的电化学势螯合钙(Ca 2+),允许它们塑造细胞内的Ca 2+的细胞内信号传导。我们描述了使用荧光染料和共聚焦显微镜同时测量线粒体的Ca 2+摄取和ΔΨ 微米活细胞的方法。

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