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Chapters
- 00:05Title
- 00:49Live Cell Imaging
- 02:46Image Analysis
- 07:08Results: Representative AT1aR-GFP Expression and Analyses
- 08:46Conclusion
Here we present a protocol to image cells expressing green fluorescent protein-tagged angiotensin type 1a receptors during endocytosis initiated by angiotensin II treatment. This technique includes labeling lysosomes with a second fluorescent marker, and then utilizing software to analyze the co-localization of receptor and lysosome in three dimensions over time.
Tags
Live Cell Imaging3D AnalysisAngiotensin Receptor Type 1aTraffickingTransfected Human Embryonic Kidney CellsConfocal MicroscopyQuantitative Spatial And Temporal EvidenceAngiotensin Two TreatmentSubcellular ProcessingY Type ReceptorMutant ReceptorInternalization ProcessLigand StimulationNumerical Aperture ObjectiveImmersion OilZ StackXYZT ModeLigand Stimulation3D Image QuantificationGFP Tagged Receptor VesicleHole Cell IntensitiesExtended Focus ModeFreestyle Region Tool
