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Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research
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JoVE 杂志 免疫与感染
Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research

Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research

DOI:
10.3791/55206-v

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09:42 min

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April 19, 2017

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, ,
1Graduate Institute of Immunology,National Taiwan University College of Medicine

Chapters

  • 00:05Title
  • 00:40Large Scale Preparation of Endotoxin-free Plasmid DNA
  • 01:36Acufection Procedure and Post-operative Care
  • 03:17Imiquimod Treatment of IL-15ΔE7-acufected Skin
  • 04:06Mouse Skin Homogenation
  • 05:20Immunohistochemical Staining of the Skin Section
  • 07:23Results: Acufection Enables an Efficient Gene Trasfer While Inducing Minimal Skin Damage
  • 08:37Conclusion

Summary

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This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.

Tags

DNA DeliveryAcufectionSkin ResearchCutaneous InflammationPsoriasisPlasmid DNABacterial CultureLB MediumEndotoxin-free Plasmid PreparationPBSDepilatory CreamEthanol DisinfectantAcupuncture ApparatusPlasmid DNA ExpressionAnesthesiaHeating PadMouse Monitoring

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