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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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生物学
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JoVE 杂志 生物学
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

DOI:
10.3791/55215-v

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08:04 min

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March 12, 2017

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, ,
1Institut Curie, INSERM U1196,Campus Universitaire d’Orsay, 2Institut Pasteur, Trypanosome Cell Biology Unit, Department of Parasites & Insect Vectors,INSERM U1201

Chapters

  • 00:05Title
  • 00:44Sample Preparation and Sectioning
  • 04:14Sample Imaging and Image Processing
  • 06:30Results: Through-focal Imaging of Trypanosoma brucei
  • 07:22Conclusion

Summary

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This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.

Tags

Scanning Transmission Electron TomographyThick Biological Samples3D ReconstructionStructural BiologyCell Culture SampleParaformaldehydeGluteraldehydeOsmium TetroxideUranyl AcetateEthanolEpoxy ResinPolymerizationSpecimen Holder

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