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单细胞基因表达谱使用流式细胞仪和qPCR内部标准
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

单细胞基因表达谱使用流式细胞仪和qPCR内部标准

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10:50 min

February 25, 2017

DOI:

10:50 min
February 25, 2017

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Summary

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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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