Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Joshua R. Porter; William G. Telford; Eric Batchelor

doi:10.3791/55219

Journal

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遗传学

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单细胞基因表达谱使用流式细胞仪和qPCR内部标准

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

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遗传学

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

单细胞基因表达谱使用流式细胞仪和qPCR内部标准

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16,354 Views

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10:50 min

•

February 25, 2017

DOI:

10.3791/55219-v

DOI:

10.3791/55219-v

•

10:50 min

February 25, 2017

•

6 Views

Joshua R. Porter¹, William G. Telford², Eric Batchelor¹

¹Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, ²Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

成績單

Summary

We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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单细胞基因表达谱使用流式细胞仪和qPCR内部标准

成績單

Automatically generated

Summary

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