Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Development of a Refined Protocol for…
JoVE Journal
Neuroscience
Author Produced

需要订阅 JoVE 才能查看此 内容.

JoVE Journal Neuroscience
Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
Journal (Neuroscience)
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
An In Ovo Model for Testing Insulin-mimetic Compounds
Journal (Neuroscience)
An In Ovo Model for Testing Insulin-mimetic Compounds
 

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Article DOI: 10.3791/55220-v • 12:45 min • August 12th, 2017
August 12th, 2017 •
1, 1, 1, 1, 1, *1, *1
1Neural Stem Cell Institute
* These authors contributed equally

章节

总结概括
Automatic Translation

Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.

Tags

Trans-scleral Subretinal Transplantation Refined Protocol Human Retinal Pigment Epithelial Cells Rat Eyes Reproducible Technique Minimal Risk Retina Damage Temporal Conjunctiva Limbus Sclera Beveled 31-gauge Insulin Needle Pilot Hole Beveled 33-gauge Injection Needle Syringe Specific Angle And Depth Injection Hole Self Seals Degenerative Retinopathies Age-related Macular Degeneration AMD Retinal Pigmented Epithelial Cells Loss Retinal Fluororeceptor Dysfunction
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter