Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Siamak Djafarzadeh; Stephan Mathias Jakob

doi:10.3791/55251

Journal

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生物学

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骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

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生物学

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JoVE 杂志生物学

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动

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18,015 Views

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10:25 min

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March 08, 2017

DOI:

10.3791/55251-v

DOI:

10.3791/55251-v

•

10:25 min

March 08, 2017

•

4 Views

Siamak Djafarzadeh¹, Stephan Mathias Jakob¹

¹Department of Intensive Care Medicine,Inselspital, Bern University Hospital

成績單

Summary

Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.