Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sarah Caughlin; Dae Hee Park; Ken K-C Yeung; David F. Cechetto; Shawn N. Whitehead

doi:10.3791/55254

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

DAN矩阵升华为神经节苷脂对大鼠脑组织的检测和可视化MALDI成像质谱

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08:36 min

March 23, 2017

DOI: 10.3791/55254-v

Sarah Caughlin¹, Dae Hee Park¹, Ken K-C Yeung², David F. Cechetto¹, Shawn N. Whitehead¹

¹Department of Anatomy and Cell Biology,The University of Western Ontario, ²Department of Chemistry and Biochemistry,The University of Western Ontario

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提出了一种使用 MALDI 成像质谱法将 DAN 基质升华到大鼠脑组织上以检测神经节苷铛苷的方案。

该升华方案的总体目标是提供详细、易于遵循的样品制备过程，用于 MALDI 成像质谱实验中神经节苷脂的检测。这种方法可以帮助回答神经生物学领域的关键问题，包括神经节苷脂在健康大脑和脑病理学中的解剖分布和功能。该技术的主要优点是它可以适应许多脂质种类的高质量成像，对方案几乎没有改变。

要开始此过程，请从大鼠中提取脑组织并按照文本协议中的说明准备低温恒温器。然后将组织放入低温恒温器中。将组织切片至所需的解剖位置。

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神经科学第121 升华 MALDI成像质谱神经节苷脂 DAN矩阵脑膜脂