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JoVE Journal
Immunology and Infection
饲养肩突硬蜱,黑腿蜱:饲养对小鼠未成熟阶段
饲养肩突硬蜱,黑腿蜱:饲养对小鼠未成熟阶段
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

饲养肩突硬蜱,黑腿蜱:饲养对小鼠未成熟阶段

Full Text
12,838 Views
06:00 min
May 8, 2017

DOI: 10.3791/55286-v

Andrew B. Nuss1, Manoj G. Mathew2, Monika Gulia-Nuss2

1Department of Agriculture, Nutrition, and Veterinary Sciences,University of Nevada, Reno, 2Department of Biochemistry and Molecular Biology,University of Nevada, Reno

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Please note that some of the translations on this page are AI generated. Click here for the English version.

健康实验室饲养蜱的生产对蜱生物学和蜱虫病原体相互作用的研究至关重要。在这里,我们展示了一种简单的方案,用于不成熟的蜱喂养,其成本效益和对小鼠的压力较小。

这种蜱虫喂养方案的总体目标是通过提高血液喂养的蜱虫的存活率来提高现有标准,同时减轻对啮齿动物宿主的压力。除了简化蜱虫群饲养外,这种方法还提供了一种更简单的方法,可以在一个收集日内收集血液喂养的蜱虫数量,这对于回答宏观生物学领域的关键生物学问题至关重要。这种技术的主要优点是蜱虫不会暴露在含有小鼠排泄物的水中,从而降低蜱虫死亡率。

此外,可以在一天内收集蜱虫,这减轻了老鼠的压力。为了容纳用于计数的蜱虫小瓶,请制作一个允许最小逸出的容器。在塑料容器的顶部边缘贴上双面胶带。

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