Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Hung-Ju Chiang; Sih-Ling Yeh; Chien-Chung Peng; Wei-Hao Liao; Yi-Chung Tung

doi:10.3791/55292

Journal

/

生物工程

/

细胞迁移研究在垂直化学和氧梯度聚二甲基硅氧烷的聚碳酸酯微流体装置

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

JoVE 杂志
生物工程

This content is Free Access.

JoVE 杂志生物工程

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

细胞迁移研究在垂直化学和氧梯度聚二甲基硅氧烷的聚碳酸酯微流体装置

Please note that all translations are automatically generated. Click here for the English version.

14,087 Views

•

11:23 min

•

February 23, 2017

DOI:

10.3791/55292-v

DOI:

10.3791/55292-v

•

11:23 min

February 23, 2017

•

6 Views

Hung-Ju Chiang¹, Sih-Ling Yeh¹, Chien-Chung Peng¹, Wei-Hao Liao¹, Yi-Chung Tung¹

¹Research Center for Applied Sciences,Academia Sinica

成績單

Summary

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.