液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法
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April 17, 2018
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在这里, 我们描述了一种色谱检测, 再加上多肽前体离子迁移分离的高分辨率 (~ 3万) MS-肽片段的检测, 用于定量测定单克隆抗体中的尖肽标准消化。
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Cite this Article
Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
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