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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Article DOI: 10.3791/55414-v • 08:06 min • February 1st, 2018
February 1st, 2018 •
*1, *1, 2, 1, 1, 1, 1, 3, 1, 4
1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group
* These authors contributed equally

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In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

Tags

Conductimetric Biosensor Assay Biomolecular Interactions Beta-lactamase Technology Antibody Antigen Interactions Chimeric Beta-lactamase Protein Proton Release Immunosensors Sensory Assays Protein Sample Preparation Solution Preparation Electrode Preparation Solution Electrode Regeneration Solution
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