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肽扫描辅助一个单克隆抗体识别的线性B细胞表位的鉴定
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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JoVE 杂志 免疫与感染
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

肽扫描辅助一个单克隆抗体识别的线性B细胞表位的鉴定

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9,398 Views

08:09 min

March 24, 2017

DOI:

08:09 min
March 24, 2017

9372 Views
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Summary

Automatically generated

Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.

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