Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Jonas Gross; Nicolas Wirth; Ingrid Tessmer

doi:10.3791/55501

Journal

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遗传学

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原子力显微镜检查DNA损伤识别核苷酸切除修复

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遗传学

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JoVE 杂志遗传学

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

原子力显微镜检查DNA损伤识别核苷酸切除修复

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9,331 Views

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10:59 min

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May 24, 2017

DOI:

10.3791/55501-v

DOI:

10.3791/55501-v

•

10:59 min

May 24, 2017

•

1 Views

Jonas Gross*¹, Nicolas Wirth*¹, Ingrid Tessmer¹

¹Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

成績單

Summary

Here, the study of different DNA lesion recognition approaches via single molecule AFM imaging is demonstrated with the nucleotide excision repair system as an example. The procedures of DNA and protein sample preparations and experimental as well as analytical details for the AFM experiments are described.