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Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
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生物工程
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JoVE 杂志 生物工程
Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation

Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation

DOI:
10.3791/55584-v

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08:24 min

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May 22, 2017

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,
1Department of Pharmaceutical Sciences, College of Pharmacy,University of North Texas Health Science Center

Chapters

  • 00:05Title
  • 01:02Preparation of Nerve Growth Factor High-density Lipoprotein (NGF HDL) Mimicking Nanoparticles
  • 02:39Characterization of NGF HDL-mimicking Nanoparticles
  • 04:13In Vitro Release of NGF HDL-mimicking Nanoparticles
  • 05:32Results: Characterization of NGF HDL-mimicking Nanoparticles
  • 07:26Conclusion

Summary

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Simple homogenization was used to prepare novel, high-density, lipoprotein-mimicking nanoparticles to encapsulate nerve growth factor. Challenges, detailed protocols for nanoparticle preparation, in vitro characterization, and in vivo studies are described in this article.

Tags

Nanoparticle PreparationHDL-mimicking NanoparticlesNerve Growth Factor EncapsulationHomogenizationPhospholipidsApolipoprotein A-1Particle CharacterizationGel Filtration ChromatographyEntrapment Efficiency

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