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JoVE Journal Genetics
Ved hjelp av en Fluorescent PCR-kapillar-gelelektroforese teknikken for å genotype CRISPR / Cas9-mediert knockout-mutanter i en high-throughput-format
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Ved hjelp av en Fluorescent PCR-kapillar-gelelektroforese teknikken for å genotype CRISPR / Cas9-mediert knockout-mutanter i en high-throughput-format

Article DOI: 10.3791/55586-v • 08:25 min • April 8th, 2017
April 8th, 2017 •
1, 1, 1, 1
1Cancer & Stem Cell Biology Programme, Duke-NUS Medical School

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Den genotyping teknikken beskrevet her, som fluorescerende par polymerase-kjedereaksjon (PCR) for å kapillært gelelektroforese, gjør det mulig for high-throughput genotyping av nuklease-mediert knockout kloner. Det omgår begrensninger overfor andre genotype-teknikker og er mer kostnadseffektivt enn sekvenseringsmetoder.

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Genetics Genome redigering knockout innsetting / sletting mutasjons high-throughput screening direkte lyse multiplexable
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