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Medicine
评估在鼠标模型中执行唤醒膀胱测压的过程
评估在鼠标模型中执行唤醒膀胱测压的过程
JoVE Journal
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JoVE Journal Medicine
Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model

评估在鼠标模型中执行唤醒膀胱测压的过程

Full Text
13,433 Views
09:31 min
May 20, 2017

DOI: 10.3791/55588-v

Travis K. Mann-Gow*1, Troy R. Larson*1, Chrissie T. Wøien2, Thomas M. Andersen2, Karl-Erik Andersson3,4, Peter Zvara1,2

1Department of Surgery,University of Vermont, 2Department of Urology and Biomedical Laboratory,University of Southern Denmark, 3Wake Forest Institute for Regenerative Medicine,Wake Forest University, 4Institute for Clinical Medicine,Aarhus University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

本研究描述了在自由移动的小鼠中进行清醒膀胱测压术的外科手术和实验技术。此外,它还提供实验证据来支持其优化和标准化。

这种显微外科手术的总体目标是将膀胱内管植入膀胱并记录清醒小鼠的下尿路功能。该方法有助于从清醒膀胱测压法中获取可重复的实验数据,以及在健康和患病小鼠模型中测试膀胱功能。该技术的主要目标是提供一种基于实验数据的标准化方法,用于在小鼠模型中进行充盈膀胱测压法。

为了准备植入管子,首先切下一根 7 厘米长的 pe10 管子,然后慢慢地将一端推向明火以产生耀斑,一旦耀斑发展,就迅速撤回管子。然后使用胶枪上的低热设置,在距离管子喇叭口端 4.5、5 和 5.5 厘米处滴三滴通用热胶。用剪刀修剪胶水气泡上的不规则之处。

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