9,847 Views
•
08:03 min
June 21, 2017
DOI:
10.3791/55638-v
介绍了通过琼脂糖凝胶阻滞测定技术生产tRNA(UUU)和tDNA(UUU)与酶TcdA复合物的分析方案。
11:01
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Related Videos
9646 Views
12:36
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
14330 Views
08:26
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
9870 Views
10:33
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
8176 Views
07:46
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
6923 Views
08:31
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
7204 Views
09:00
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
11534 Views
08:33
Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
8924 Views
10:14
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
14671 Views
07:32
Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
12446 Views
Read Article
Cite this Article
Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).
Download .ris file
Copy
Share Video
.