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测定在运动神经元样NSC-34细胞中实时测量核细胞质运输

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

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神经科学

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JoVE 杂志神经科学

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

测定在运动神经元样NSC-34细胞中实时测量核细胞质运输

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8,624 Views

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08:53 min

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May 16, 2017

DOI:

10.3791/55676-v

DOI:

10.3791/55676-v

•

08:53 min

May 16, 2017

•

2 Views

Tom Shani¹, Moshe Levy¹, Adrian Israelson^1,2

¹Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, ²The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev

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Summary

该方案描述了一种通过量化NLS-NES-GFP蛋白的核进口的实时变化来敏感地测量运动神经元样NSC-34细胞内的核胞质转运速率的方法。

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