Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Rongli Zhang; Jonathan W. Ray; Mukesh K. Jain; Shuxin Han

doi:10.3791/55728

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JoVE Journal Medicine

Ileectomy-induced Bile Overaccumulation in Mouse Intestine

回肠致胆汁积滞在小鼠肠道中

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06:55 min

August 21, 2017

DOI: 10.3791/55728-v

Rongli Zhang*^1,2,3, Jonathan W. Ray^1,2, Mukesh K. Jain^1,2, Shuxin Han*^1,2

¹Department of Medicine, Case Cardiovascular Research Institute,Case Western Reserve University, ²Harrington Heart and Vascular Institute,University Hospitals Cleveland Medical Center, ³Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine,University Hospitals Cleveland Medical Center

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小肠依赖胆汁酸的重吸收和反馈抑制肝胆汁酸合成系统稳态和健康至关重要。在此研究中，我们描述为回肠切除回肠致胆汁吸收不良，积滞，和毒性小鼠肠道中的小鼠模型。

该手术的总体目标是评估回肠切除术诱导的小鼠肠道胆汁吸收不良、过度积累和毒性。回肠疾病如克罗恩病、癌症、自身免疫性疾病或感染等表现病症是发病率和死亡率的重要来源。我们相信，这种涉及节段性回肠切除的模型的发展可用于深入了解生物学和治疗。

该技术的意义延伸到胆汁酸腹泻的治疗，因为回肠切除术可能会诱导肝脏产生胆汁酸过多，以及肠道吸收不良和过度积累。一般来说，刚接触这种方法的人会与肠道吻合术作斗争，这是这种手术中最关键和最困难的部分。首先通过捏住脚趾确认适当的镇静水平，并在小鼠的腹部涂抹脱毛霜。

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医药问题 126 回肠小鼠疾病模型组织沟通碱性成纤维细胞生长因子 15 (FGF15) 胆汁酸的重吸收短肠综合征