Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
6,477 Views
•
12:50 min
•
September 15, 2017
DOI:
成績單
Automatically generated
The overall goal of this screening assay is to characterize novel endothelial regulators involved in the inflammatory response of interest for designing new therapeutic strategies against inflammatory disorders.This method can help answer key questions in the pathology field such as defining novel endothelial-targeting drugs able to attenuate inflammatory-related diseases.The main advantage of this endothelial protocol is that describes assay technology that establishes the basis for exploring novel mechanisms involved in the regulation of the inflammatory response.Demonstrating this methodology will be Maria Angeles Higueras, a post-doc from our lab and Sandra Herranz, a technician from our lab.To begin, culture MLEC-04 cells in complete DMEM F12 medium supplemented with 10%FBS and Pen-Strep.Incubate the cells in a biological incubator at 37
Summary
Automatically generated
Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.
Read Article
Cite this Article
Higueras, M. Á., Jiménez-García, L., Herranz, S., Hortelano, S., Luque, A. Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response. J. Vis. Exp. (127), e55824, doi:10.3791/55824 (2017).
Copy