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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Article DOI: 10.3791/55870-v • 06:26 min • June 19th, 2017
June 19th, 2017 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery, University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock

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Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

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Gap Junction-dependent Transfer MiRNA 3D-FRAP Microscopy Cardiomyocytes Intercellular Signal Transaction System Visualized Quantified Spatial Temporal Resolution Isolating Cardiomyocytes Plate Cells Cell Culture Medium Trypzine Centrifuge Cells Electroporation Buffer Fluorescent MiRNA Electroporation Cuvette Electroporation Program Glass Bottom Chamber Slide
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