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05:41 min
September 11, 2017
DOI:
10.3791/55975-v
这份手稿描述了一个体外胚珠培养方法, 使受精卵和胚胎的活细胞成像的拟南芥。该方法用于在受精卵分化过程中对细胞内动力学进行可视化, 并对发育中的胚胎的胞命运规范进行研究。
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Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
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Cite this Article
Kurihara, D., Kimata, Y., Higashiyama, T., Ueda, M. In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana. J. Vis. Exp. (127), e55975, doi:10.3791/55975 (2017).
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