细菌显示文库的半自动化筛选肽亲和试剂的发现与分离结果分析
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December 06, 2017
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筛选细菌显示库是一种被证实的技术, 用于发现肽亲和试剂, 一种强有力的替代抗体。本文的半自动排序方法简化了筛选, 减少了误报的发生。在这里, 我们说明的思想过程和技术应用于评价候选人和最小化下游分析。
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Cite this Article
Sarkes, D. A., Jahnke, J. P., Stratis-Cullum, D. N. Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates. J. Vis. Exp. (130), e56061, doi:10.3791/56061 (2017).
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