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Voorbereiding en metabole kwantitatieve analyse van 3-dimensionale sferoïde co culturen van Pancreatic kankercellen en fibroblasten

Article DOI: 10.3791/56081-v • 10:51 min • August 23rd, 2017
August 23rd, 2017 •
1, 1, 2, 2, 1, 1
1Molecular Medicine Division, Translational Genomics Research Institute, 2Agilent Technologies

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Hier, wordt een methode beschreven voor de bereiding van 3-dimensionale (3D) sferoïde co cultuur van pancreatic kankercellen en fibroblasten, gevolgd door meting van metabolische functies met behulp van een extracellulaire flux-analyzer.

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Kankeronderzoek kwestie 126 alvleesklierkanker 3-dimensionale spheroïden co cultuur extracellulaire flux Bioenergetica mitochondriale stress
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