上述 秀丽隐杆线虫 排泄管是用于可视化研究的单细胞独特模型 在体内 分析 从头 极性膜生物合成。本方案描述了标准遗传学/RNAi与成像技术的结合方法,可适用于鉴定和表征调控单细胞管状发生以及顶膜和管腔生物合成的分子。
Chapters in this video
0:05
Title
1:02
Introduction to the Accompanying Publication
2:28
RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension
3:29
Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy
5:20
Imaging the C. elegans Excretory Canal using Confocal Microscopy
8:16
Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes
9:51
Conclusion
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