A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Zhe Liu; Siling Fu; Nan Tang

doi:10.3791/56114

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

通过小鼠肺切除术和假体植入测量内部肺表面积的标准方法

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08:46 min

July 26, 2017

DOI: 10.3791/56114-v

Zhe Liu^1,2, Siling Fu^2,3, Nan Tang²

¹Department of Life Sciences,Peking University, ²National Institute of Biological Sciences, Beijing, ³Graduate School of Peking Union Medical College

Please note that some of the translations on this page are AI generated. Click here for the English version.

内肺表面积（ISA）是评估肺部疾病和损伤诱导肺泡再生的肺形态和生理学的关键标准。我们在这里描述了一种标准化方法，可以最大限度地减少肺肺切除术和假体植入小鼠模型中ISA的测量偏差。

该程序的总体目标是准确测量全肺切除术和假体植入后小鼠肺的内表面积。该方法有助于分析机械力诱导的成人肺再生中肺的形态和生理功能。该技术的主要优点是准确量化肺内表面积，有望提高肺功能状态的可靠性和可重复性。

准备在插管平台上进行手术，使其腹侧可触及。然后拉出舌头，用与导尿管兼容的小动物喉镜检查声带。然后将 20 号静脉插管插管以内角轻轻插入气管。

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发育生物学第125期内肺表面积内肺容积平均线性截距小鼠肺切除术假体植入肺泡再生