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JoVE Journal Cancer Research
TIP60 短暂耗竭后乳腺上皮细胞迁移的活体影像学研究
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TIP60 短暂耗竭后乳腺上皮细胞迁移的活体影像学研究

Article DOI: 10.3791/56248-v • 08:13 min • December 7th, 2017
December 7th, 2017 •
1, 1, 1, 1,2
1Cancer Science Institute of Singapore, National University of Singapore, 2Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore

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在这里, 我们提出了实时监测细胞迁移在伤口愈合试验中使用 TIP60-depleted MCF10A 乳腺上皮细胞。在我们的协议中实现了活细胞成像技术, 使我们能够实时和跨时间地分析和可视化单细胞运动。

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癌症研究 问题 130 活细胞成像 细胞迁移 伤口愈合 TIP60 瞬态损耗 MCF10A
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