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胸腺1-gmcmp6 转基因小鼠在原代体血源皮质中的成像神经活性

Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

JoVE 杂志
神经科学

This content is Free Access.

JoVE 杂志神经科学

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

胸腺1-gmcmp6 转基因小鼠在原代体血源皮质中的成像神经活性

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11,007 Views

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07:04 min

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January 07, 2019

DOI:

10.3791/56297-v

DOI:

10.3791/56297-v

•

07:04 min

January 07, 2019

•

10982 Views

Xiaojing Lin^1,2, Tingbao Zhao³, Wenhui Xiong¹, Shaonan Wen⁴, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine ²Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region ³Department of Orthopedics, Shandong Cancer Hospital,Shandong University ⁴Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences

Summary

我们描述了一种实验程序, 测量双光窗口以上的双光窗口双侧原生体感骨 (s1) 在 thy1-gcmp6 转基因小鼠使用2光子 (2p) 显微镜在体内 .

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