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JoVE Journal Bioengineering
化重构法 (的) 对肠道干细胞和小生境细胞功能分析的研究
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化重构法 (的) 对肠道干细胞和小生境细胞功能分析的研究

Article DOI: 10.3791/56329-v • 09:38 min • November 20th, 2017
November 20th, 2017 •
1, 1, 1, 1
1Department of Pathology, Erasmus MC Cancer Institute, Erasmus University Medical Center

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肠道化文化是建立在整个棺, 不允许分析自我更新和分化的细胞特有的方式。本协议描述了对排序后的词干 (Lgr5+) 和小生境 (氏) 单元的重构, 这些细胞在进行生物化学和遗传修饰和功能分析之前, 会产生 organoids。

Tags

生物工程 问题 129 mini-gut organoids Lgr5+干细胞 氏细胞 肠道干细胞生态位 化重构法 肠道共培养
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