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Journal / Cancer Research / 单细胞下游特征的靶细胞预富集与全基因组扩增…
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Cancer Research

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JoVE Journal Cancer Research
单细胞下游特征的靶细胞预富集与全基因组扩增
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单细胞下游特征的靶细胞预富集与全基因组扩增

Article DOI: 10.3791/56394-v • 10:12 min • May 15th, 2018
May 15th, 2018 •
*1, *1, 1, 2, 3, 3, 4, 1,5, 1
1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg
* These authors contributed equally

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该协议是从非目标背景细胞的混合物中恢复和制备稀有靶细胞, 用于单细胞水平的分子遗传表征。DNA 质量等于未处理的单个细胞, 并允许单细胞应用 (筛查和靶向分析)。

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癌症研究 135 期 稀有细胞分析 单细胞分析 全基因组扩增 阵列比较基因组杂交,体内隔离装置 目标下一代测序 下一代测序免疫荧光标签
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