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发育生物学

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小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

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发育生物学

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JoVE 杂志发育生物学

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

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9,562 Views

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09:42 min

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October 12, 2017

DOI:

10.3791/56446-v

DOI:

10.3791/56446-v

•

09:42 min

October 12, 2017

•

3 Views

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core,Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience,Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

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Summary

在这里, 我们描述了一个协议的全贴装免疫荧光和图像定量容积分析早期小鼠胚胎。我们提出这项技术是一个强有力的方法, 定性和定量评估心脏结构在发展过程中, 并提出它可能会广泛适用于其他器官系统。

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