Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Xue Li; Dan D. Huang; Rui Du; Zhi J. Zhang; Chak K. Chan; Zheng X. Huang; Zhen Zhou

doi:10.3791/56465

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

利用二次 Nanoelectrospray 电离耦合高分辨质谱的实时呼吸分析

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08:23 min

March 9, 2018

DOI: 10.3791/56465-v

Xue Li*^1,2, Dan D. Huang*³, Rui Du^1,2, Zhi J. Zhang¹, Chak K. Chan³, Zheng X. Huang^1,2, Zhen Zhou^1,2

¹Institute of Mass Spectrometer and Atmospheric Environment,Jinan University, ²Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, ³School of Energy and Environment,City University of Hong Kong

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本文证明了用二次 nanoelectrospray 电离耦合高分辨质谱法对呼气的化学成分进行实时表征的一种协议。

本实验的总体目标是使用二次纳米电喷雾电离与高分辨率质谱联用来表征呼出气的化学成分。这种方法可以帮助回答医学领域的关键问题，例如疾病的早期诊断、治疗检查监测和环境暴露。该技术的主要优点是可以实时进行测量。

该技术还具有最高产量、灵敏、特异性和用户友好性。这项技术的影响已扩展到肺癌的诊断。因为在不同阶段的肺癌患者的呼气中发现了生物标志物候选者的机会。

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化学问题 133 挥发性有机化合物呼吸分析二次 nanoelectrospray 电离高分辨率质谱实时