An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a <em>Drosophila</em> Model of Neurodegeneration

Wenze Cao; Li Song; Jingjing Cheng; Na Yi; Luyi Cai; Fu-de Huang; Margaret Ho

doi:10.3791/56507

一种自动快速迭代阴性 Geotaxis 分析方法在神经的果蝇模型中的成人攀爬行为

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JoVE Journal Behavior

An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

一种自动快速迭代阴性 Geotaxis 分析方法在神经的果蝇模型中的成人攀爬行为

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06:52 min

September 12, 2017

DOI: 10.3791/56507-v

Wenze Cao*^1,2,3, Li Song*^1,2,3, Jingjing Cheng^1,2,3, Na Yi^1,2,3, Luyi Cai^1,2,3, Fu-de Huang^4,5, Margaret Ho^1,2,3

¹Research Center for Translational Medicine,Tongji University School of Medicine, ²Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital,Tongji University School of Medicine, ³Department of Anatomy and Neurobiology,Tongji University School of Medicine, ⁴Shanghai Advanced Research Institute, University of Chinese Academy of Sciences,Chinese Academy of Sciences, ⁵Sino-Danish College, University of Chinese Academy of Sciences,Chinese Academy of Sciences

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此 step-by 步骤协议使用自动缸系统来分析果蝇的负 geotaxis 行为, 它承载数以百计的苍蝇并使其动作与电动机同步。在同步时, 采用自行设计的 RflyDetection 软件对飞行负 geotaxis 行为进行检测、数字记录和分析。

这种自动化快速迭代阴性趋地性测定的总体目标是分析与神经退行性相关的果蝇成体攀爬缺陷。这种方法可以帮助回答神经科学领域的关键问题，例如与神经退行性变相关的运动缺陷机制。这种技术的主要优点是可以在自动化和高证明专业中分析飞蝇攀爬活动。

该技术的意义延伸到神经退行性疾病的治疗和诊断，因为神经退行性疾病通常与运动能力的进行性丧失和寿命缩短以及年龄依赖性神经退行性变有关。我们首先想到了这种方法。我们想以更准确的方式测量果蝇成人的运动活动。

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行为问题 127 果蝇成人攀登帕金森病年龄依赖性神经负 geotaxis 自发行为