11,761 Views
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08:16 min
April 02, 2018
DOI:
10.3791/56618-v
该协议描述了一个平台, 用于制造可变尺寸的自组装组织环使用定制的 3 d 印刷塑料模具。在 3 d-印刷模中固化了该底片;然后, 琼脂糖被投在固化的中和阴性。细胞被播种到产生的琼脂糖阱中, 它们聚集成组织环。
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Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
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Cite this Article
Strobel, H. A., Calamari, E. L., Alphonse, B., Hookway, T. A., Rolle, M. W. Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds. J. Vis. Exp. (134), e56618, doi:10.3791/56618 (2018).
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